Structural insight into processive human mitochondrial DNA synthesis and disease-related polymerase mutations. |
Poxviruses replicate in the host cytoplasm using a processive polymerase composed of several viral proteins. |
The approach is based on eavesdropping on a single DNA polymerase molecule working in a continuous, processive manner. |
As a result, extension rates better reflect the kinetics seen in PCR with processive extension of a defined template for more reproducible activity measurements. |